Validation Data Gallery
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Tested Applications
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-15697 targets PRM1 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8323 Product name: Recombinant human PRM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-51 aa of BC003673 Sequence: MARYRCCRSQSRSRYYRQRQRSRRRRRRSCQTRRRAMRCCRPRYRPRCRR 相同性解析による交差性が予測される生物種 |
| Full Name | PRM1 |
| Calculated molecular weight | 51 aa, 6 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC003673 |
| Gene Symbol | PRM1 |
| Gene ID (NCBI) | 5619 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity Purified |
| UNIPROT ID | P04553 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRM1 is a 51 amino acid protein. PRM1 substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823 )
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 PRM1 antibody CL594-15697 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
