Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, MCF-7 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17985-1-AP targets PRKX/PRKY in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12404 Product name: Recombinant human PRKY protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-277 aa of BC074852 Sequence: MEAPGPAQAAAAESNSREVTEDAADWAPALCPSPEARSPEAPAYRLQDCDALVTMGTGTFGRVHLVKEKTAKHFFALKVMSIPDVIRRKQEQHVHNEKSVLKEVSHPFLIRLFWTWHEERFLYMLMEYVPGGELFSYLRNRGHFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILLDRDGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILAGKLYFPRHLDFHVKTGRMM 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase, Y-linked |
| Calculated molecular weight | 277 aa, 32 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC074852 |
| Gene Symbol | PRKY |
| Gene ID (NCBI) | 5616 |
| RRID | AB_3669286 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRKY is a member of the cAMP-dependent serine-threonine protein kinase gene family, which appears to encode a protein related to the catalytic subunit of the cAMP-dependent protein kinases, which are key players in the cellular responses to the second messenger cAMP. A chromosomal aberration involving PRKY is a cause of sex reversal disorder. As a homolog with PRKX, the antibody can recognize endogenous levels of PRKY and PRKX.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRKX/PRKY antibody 17985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |