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- KD/KO Validated
PACT Polyclonal antibody
PACT Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10771-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, C2C12 cells, PC-12 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10771-1-AP targets PACT in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PACT fusion protein Ag1214 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase, interferon-inducible double stranded RNA dependent activator |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 31-34 kDa |
| GenBank accession number | BC009470 |
| Gene symbol | PRKRA |
| Gene ID (NCBI) | 8575 |
| RRID | AB_2170496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PACT antibody 10771-1-AP | Download protocol |
| IHC protocol for PACT antibody 10771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
EMBO Rep The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development. | ||
Nucleic Acids Res Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA. | ||
PLoS Pathog KSHV inhibits stress granule formation by viral ORF57 blocking PKR activation. | ||
J Biol Chem PACT Establishes a Post-transcriptional Brake on Mitochondrial Biogenesis by Promoting the Maturation of miR-181c. |
