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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PRKG2 Polyclonal antibody (55138-1-AP)

    WB analysis of mouse brain using 55138-1-AP (same clone as 55138-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRKG2 Polyclonal antibody (55138-1-AP)

    WB analysis using 55138-1-AP (same clone as 55138-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRKG2 Polyclonal antibody (55138-1-AP)

    IP experiment of mouse brain using 55138-1-AP (same clone as 55138-1-PBS)

    IP result of anti-PRKG2 (IP:55138-1-AP, 4ug; Detection:55138-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRKG2 Polyclonal antibody (55138-1-AP)

    IHC staining of human colon using 55138-1-AP (same clone as 55138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRKG2 Polyclonal antibody (55138-1-AP)

    IHC staining of human colon using 55138-1-AP (same clone as 55138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PRKG2 Polyclonal antibody (55138-1-AP)

    IHC staining of human lung using 55138-1-AP (same clone as 55138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PRKG2 Polyclonal antibody (55138-1-AP)

    IHC staining of human lung using 55138-1-AP (same clone as 55138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using PRKG2 Polyclonal antibody (55138-1-AP)

    IF Staining of C6 using 55138-1-AP (same clone as 55138-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PRKG2 antibody (55138-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using PRKG2 Polyclonal antibody (55138-1-AP)

    IF Staining of C6 using 55138-1-AP (same clone as 55138-1-PBS)

    Immunofluorescent analysis of C6 cells using 55138-1-AP (PRKG2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 55138-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PRKG2 Polyclonal antibody, PBS Only

PRKG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 55138-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

cGK2, cGKII, cGMP dependent protein kinase 2, cGMP-dependent protein kinase 2, cGMP-dependent protein kinase II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55138-1-PBS targets PRKG2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name protein kinase, cGMP-dependent, type II
Calculated molecular weight 87 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberNM_006259
Gene Symbol PRKG2
Gene ID (NCBI) 5593
RRIDAB_10804353
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13237
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRKG2, also named as PRKGR2 and cGK2, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and cGMP subfamily. It catalyses the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to PRKG2.

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