Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, mouse lung tissue, rat lung tissue, mouse brain tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85983-1-RR targets PRKG1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PRKG1 fusion protein Ag16333 相同性解析による交差性が予測される生物種 |
Full Name | protein kinase, cGMP-dependent, type I |
Calculated molecular weight | 686 aa, 78 kDa |
Observed molecular weight | 65-78 kDa |
GenBank accession number | BC127090 |
Gene Symbol | PRKG1 |
Gene ID (NCBI) | 5592 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13976 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRKG1(cGMP-dependent protein kinase 1) is also named as cGK1, PRKG1B, PRKGR1A, PRKGR1B and belongs to the cGMP subfamily. It serves as an integral component of second messenger signaling in a number of biological contexts including cell differentiation, memory, and vasodilation (PMID:21893290). Its activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells (PMID:20060325). PRKG1 has 2 isoforms produced by alternative splicing with the molecular weight of 76 kDa and 78 kDa. The autophosphorylation can increase the kinase activity and it can form a monomer with the molecular weight of 65 kDa that is produced by proteolytic cleavage. SDS-PAGE revealed that proteolysis generated two different monomers with molecular masses of 70 and 68 kDa of CGK1-beta(PMID:8702828).
Protocols
Product Specific Protocols | |
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WB protocol for PRKG1 antibody 85983-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |