Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, NIH/3T3 cells, C2C12 cells, Raji cells, Ramos cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | DU 145 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
12785-1-AP targets PRKD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3499 Product name: Recombinant human PRKD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC030706 Sequence: MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSTDEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDID 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase D3 |
| Calculated molecular weight | 611aa,68 kDa; 100 kDa; 890aa |
| Observed molecular weight | 68 kDa, 100 kDa |
| GenBank accession number | BC030706 |
| Gene Symbol | PRKD3 |
| Gene ID (NCBI) | 23683 |
| RRID | AB_2877881 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94806 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein kinase D 3 (PRKD3), a serine/threonine protein kinase, functions as a crucial regulator across numerous cancer types. High mRNA and protein levels of PRKD3 were reported to be associated with multiple tumor nodules, vascular infiltration, advanced AJCC staging, and poor prognosis in patients with hepatocellular carcinoma (HCC) after liver resection, highlighting the oncogenic function of PRKD3 (PMID: 39678603, 41980898).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| IHC protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| IP protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| WB protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Mol Med Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling | ||
NAR Cancer Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs | ||
Cancer Rep (Hoboken) PRKD3 Overexpression May Improve Survival and Suppresses Proliferation in Colorectal Cancer. |