Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12785-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 12785-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12785-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12785-1-AP
IP result of anti-PRKD3 (IP:12785-1-AP, 4ug; Detection:12785-1-AP 1:300) with HEK-293 cells lysate 2800ug.
IHC staining of human breast cancer using 12785-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12785-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12785-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12785-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRKD3 Polyclonal antibody

PRKD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12785-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Synonyms

EPK2, PKC NU, PKD3, PRKCN, PRKD3, Protein kinase C nu type, protein kinase D3, Protein kinase EPK2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	DU 145 cells, HeLa cells, NIH/3T3 cells, C2C12 cells, Raji cells, Ramos cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

12785-1-AP targets PRKD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3499 Product name: Recombinant human PRKD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC030706 Sequence: MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSTDEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDID 相同性解析による交差性が予測される生物種
Full Name	protein kinase D3
Calculated molecular weight	611aa,68 kDa; 100 kDa; 890aa
Observed molecular weight	68 kDa, 100 kDa
GenBank accession number	BC030706
Gene Symbol	PRKD3
Gene ID (NCBI)	23683
RRID	AB_2877881
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94806
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for PRKD3 antibody 12785-1-AP	Download protocol
IP protocol for PRKD3 antibody 12785-1-AP	Download protocol
WB protocol for PRKD3 antibody 12785-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF,IP	Exp Mol Med Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling Authors - Chunlei Liu View Article
human	WB	NAR Cancer Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs Authors - Spencer Arnesen View Article

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Validation Data Gallery

WB analysis using 12785-1-AP

WB analysis of DU 145 using 12785-1-AP