Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 10860-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10860-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10860-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 10860-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 40x lens).
IHC staining of mouse eye using 10860-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 10860-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10860-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10860-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 10860-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PKC Zeta antibody (10860-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10860-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, NIH/3T3 cells
Positive IHC detected in	human breast cancer tissue, mouse cerebellum tissue, mouse eye tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

10860-1-AP targets PKC Zeta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1310 Product name: Recombinant human PRKCZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 244-592 aa of BC014270 Sequence: SQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIKSHAFFRSIDWDLLEKKQALPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDAIKRIDQSEFEGFEYINPLLLSTEESV 相同性解析による交差性が予測される生物種
Full Name	protein kinase C, zeta
Calculated molecular weight	80 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC014270
Gene Symbol	PKC Zeta
Gene ID (NCBI)	5590
RRID	AB_2300348
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q05513
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for PKC Zeta antibody 10860-1-AP	Download protocol
IHC protocol for PKC Zeta antibody 10860-1-AP	Download protocol
WB protocol for PKC Zeta antibody 10860-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Sci Rep aPKCζ-dependent Repression of Yap is Necessary for Functional Restoration of Irradiated Salivary Glands with IGF-1. Authors - Alejandro M Chibly KO Validated View Article
human,mouse	WB,IHC	Cancers (Basel) Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma Authors - Hao-Fan Wang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 10860-1-AP

WB analysis of NIH/3T3 using 10860-1-AP

IHC staining of human breast cancer using 10860-1-AP