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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PRKCDBP Polyclonal antibody (16250-1-AP)

    WB analysis of HeLa using 16250-1-AP (same clone as 16250-1-PBS)

    WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRKCDBP Polyclonal antibody (16250-1-AP)

    WB analysis using 16250-1-AP (same clone as 16250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PRKCDBP Polyclonal antibody (16250-1-AP)

    WB analysis of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using PRKCDBP Polyclonal antibody (16250-1-AP)

    IP experiment of A549 using 16250-1-AP (same clone as 16250-1-PBS)

    IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 1120 ug. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRKCDBP Polyclonal antibody (16250-1-AP)

    IHC staining of human colon using 16250-1-AP (same clone as 16250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PRKCDBP Polyclonal antibody (16250-1-AP)

    IF Staining of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PRKCDBP Polyclonal antibody, PBS Only

PRKCDBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16250-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16250-1-PBS targets PRKCDBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9209

Product name: Recombinant human PRKCDBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-261 aa of BC011585

Sequence: MRESALEPGPVPEAPAGGPVHAVTVVTLLEKLASMLETLRERQGGLARRQGGLAGSVRRIQSGLGALSRSHDTTSNTLAQLLAKAERVSSHANAAQERAVRRAAQVQRLEANHGLLVARGKLHVLLFKEEGEVPASAFQKAPEPLGPADQSELGPEQLEAEVGESSDEEPVESRAQRLRRTGLQKVQSLRRALSGRKGPAAPPPTPVKPPRLGPGRSAEAQPEAQPALEPTLEPEPPQDTEEDPGRPGAAEEALLQMESVA

相同性解析による交差性が予測される生物種
Full Name protein kinase C, delta binding protein
Calculated molecular weight 261 aa, 28 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC011585
Gene Symbol PRKCDBP
Gene ID (NCBI) 112464
RRIDAB_2171894
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969G5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta

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