Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 10142-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 10142-2-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10142-2-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10142-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10142-2-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10142-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10142-2-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa cells using 10142-2-AP
IP result of anti-PRKAR2A (10142-2-AP for IP and Detection).
IP experiment of HeLa using 10142-2-AP
IP result of anti-PRKAR2A (IP:10142-2-AP, 4ug; Detection:10142-2-AP 1:2000) with HeLa cells lysate 1320 ug.
IHC staining of mouse colon using 10142-2-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10142-2-AP (PRKAR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 10142-2-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRKAR2A antibody (10142-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PRKAR2A Polyclonal antibody

PRKAR2A Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 10142-2-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

cAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells, human testis tissue
Positive IP detected in	HeLa cells, HeLa cells
Positive IHC detected in	mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

10142-2-AP targets PRKAR2A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0203 Product name: Recombinant human PRKAR2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-382 aa of BC002763 Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ 相同性解析による交差性が予測される生物種
Full Name	protein kinase, cAMP-dependent, regulatory, type II, alpha
Calculated molecular weight	43 kDa
Observed molecular weight	46-52 kDa
GenBank accession number	BC002763
Gene Symbol	PRKAR2A
Gene ID (NCBI)	5576
RRID	AB_2168236
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13861
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.

Protocols

Product Specific Protocols
IF protocol for PRKAR2A antibody 10142-2-AP	Download protocol
IP protocol for PRKAR2A antibody 10142-2-AP	Download protocol
WB protocol for PRKAR2A antibody 10142-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease Authors - Chandana Thimme Gowda View Article
human	WB	Cancer Lett Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma. Authors - Furong Huang KD Validated View Article
rat	WB,IHC	Life Sci TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats. Authors - Haochen Wang View Article
mouse,human	WB,IP	Cell Rep STK25 inhibits PKA signaling by phosphorylating PRKAR1A Authors - Xiaokan Zhang View Article
mouse	WB	J Transl Med Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease Authors - Qinglong Guo View Article
human	WB	J Transl Med F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration Authors - Wuping Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10142-2-AP

WB analysis of human testis using 10142-2-AP

WB analysis of MCF-7 using 10142-2-AP

WB analysis of HeLa using 10142-2-AP

WB analysis of K-562 using 10142-2-AP

WB analysis of mouse testis using 10142-2-AP

WB analysis of PC-3 using 10142-2-AP

IP experiment of HeLa cells using 10142-2-AP

IP experiment of HeLa using 10142-2-AP

IHC staining of mouse colon using 10142-2-AP

IF Staining of mouse testis using 10142-2-AP

PRKAR2A Polyclonal antibody

PRKAR2A Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease

Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma.

TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats.

STK25 inhibits PKA signaling by phosphorylating PRKAR1A

Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease

F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration