Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
20358-1-PBS targets PRKAR1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14216 Product name: Recombinant human PRKAR1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC036285 Sequence: MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAI 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) |
| Calculated molecular weight | 381 aa, 43 kDa |
| Observed molecular weight | 43-45 kDa |
| GenBank accession number | BC036285 |
| Gene Symbol | PRKAR1A |
| Gene ID (NCBI) | 5573 |
| RRID | AB_10697659 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10644 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.