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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKACB Recombinant antibody (85716-2-RR)

    WB analysis using 85716-2-RR (same clone as 85716-2-PBS)

    Fetal human brain tissue lysates were subjected to SDS PAGE followed by western blot with 85716-2-RR (PRKACB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85716-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRKACB Recombinant antibody (85716-2-RR)

    WB analysis using 85716-2-RR (same clone as 85716-2-PBS)

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 85716-2-RR (PRKACB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85716-2-PBS in a different storage buffer formulation.

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PRKACB Recombinant antibody, PBS Only

PRKACB Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85716-2-PBS
Clone No.242947A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:2.7.11.11, PKA C beta, PKA C-beta, PKACB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85716-2-PBS targets PRKACB in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, catalytic, beta
Calculated molecular weight 46 kDa
Observed molecular weight 36-46 kDa
GenBank accession numberNM_002731
Gene Symbol PRKACB
Gene ID (NCBI) 5567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22694
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.