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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PRKACA Monoclonal antibody (67491-1-Ig)

    WB analysis of HeLa using 67491-1-Ig (same clone as 67491-1-PBS)

    WB result of PRKACA antibody (67491-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRKACA Monoclonal antibody (67491-1-Ig)

    WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67491-1-Ig (PRKACA antibody) at dilution of 1:2300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human prostate cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human prostate cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PRKACA Monoclonal antibody, PBS Only

PRKACA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67491-1-PBS
Clone No.4G10D7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Synonyms

PKA C alpha, PKACA, PRKACA

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67491-1-PBS targets PRKACA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26400

Product name: Recombinant human PRKACA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 162-351 aa of BC039846

Sequence: DLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Calculated molecular weight 41 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC039846
Gene Symbol PRKACA
Gene ID (NCBI) 5566
RRIDAB_2882716
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17612
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus.

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