Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10773-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Sp2/0 using 10773-1-AP
Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10773-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Sp2/0 using 10773-1-AP
Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10773-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10773-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10773-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10773-1-AP
IP result of anti-PRIM1 (IP:10773-1-AP, 4ug; Detection:10773-1-AP 1:300) with HeLa cells lysate 2800ug.
IHC staining of human lymphoma using 10773-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 10773-1-AP (PRIM1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human thyroid cancer using 10773-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10773-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10773-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRIM1 antibody (10773-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PRIM1 Polyclonal antibody

PRIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10773-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

p49, EC:2.7.7.102, DNA primase small subunit, DNA primase 49 kDa subunit

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Sp2/0 cells, K-562 cells, HepG2 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lymphoma tissue, human thyroid cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 2 publications below

Product Information

10773-1-AP targets PRIM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRIM1 fusion protein Ag1124 相同性解析による交差性が予測される生物種
Full Name	primase, DNA, polypeptide 1 (49kDa)
Calculated molecular weight	50 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC005266
Gene Symbol	PRIM1
Gene ID (NCBI)	5557
RRID	AB_2237549
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49642
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subnuit of this complex.

Protocols

Product Specific Protocols
WB protocol for PRIM1 antibody 10773-1-AP	Download protocol
IHC protocol for PRIM1 antibody 10773-1-AP	Download protocol
IF protocol for PRIM1 antibody 10773-1-AP	Download protocol
IP protocol for PRIM1 antibody 10773-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol 53BP1-shieldin-dependent DSB processing in BRCA1-deficient cells requires CST-Polα-primase fill-in synthesis. Authors - Zachary Mirman View Article
human	WB	Nucleic Acids Res CST does not evict elongating telomerase but prevents initiation by ssDNA binding. Authors - Arthur J Zaug View Article
mouse		Cell Rep A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice. Authors - Liam C Hunt View Article
human	WB	DNA Repair (Amst) Expression of BRCA1, BRCA2, RAD51, and other DSB repair factors is regulated by CRL4WDR70. Authors - Zachary Mirman View Article
	IHC	PLoS One Optimization of the chicken chorioallantoic membrane assay as reliable in vivo model for the analysis of osteosarcoma. Authors - Pierre Kunz View Article
human	WB	bioRxiv RTF2 controls replication repriming and ribonucleotide excision at the replisome Authors - Brooke A Conti View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10773-1-AP

WB analysis of Sp2/0 using 10773-1-AP

WB analysis of HeLa using 10773-1-AP

WB analysis of Sp2/0 using 10773-1-AP

WB analysis of K-562 using 10773-1-AP

WB analysis of HeLa using 10773-1-AP

WB analysis of K-562 using 10773-1-AP

IP experiment of HeLa using 10773-1-AP

IHC staining of human lymphoma using 10773-1-AP

IHC staining of human thyroid cancer using 10773-1-AP

IHC staining of human thyroid cancer using 10773-1-AP

IF Staining of HeLa using 10773-1-AP

PRIM1 Polyclonal antibody

PRIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

53BP1-shieldin-dependent DSB processing in BRCA1-deficient cells requires CST-Polα-primase fill-in synthesis.

CST does not evict elongating telomerase but prevents initiation by ssDNA binding.

A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.

Expression of BRCA1, BRCA2, RAD51, and other DSB repair factors is regulated by CRL4WDR70.

Optimization of the chicken chorioallantoic membrane assay as reliable in vivo model for the analysis of osteosarcoma.

RTF2 controls replication repriming and ribonucleotide excision at the replisome