Validation Data Gallery
Tested Applications
| Positive IP detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25736-1-AP targets PRIC285 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22719 Product name: Recombinant human PRIC285 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1733-2080 aa of BC150283 Sequence: DTRLQRGELFSREDLVWYKKVLWEARKFELDRHEVILCTCSCAASASLKILDVRQILVDEAGMATEPETLIPLVQFPQAEKVVLLGDHKQLRPVVKNERLQNLGLDRSLFERYHEDAHMLDTQYRMHEGICAFPSVAFYKSKLKTWQGLRRPPSVLGHAGKESCPVIFGHVQGHERSLLVSTDEGNENSKANLEEVAEVVRITKQLTLGRTVEPQDIAVLTPYNAQASEISKALRREGIAGVAVSSITKSQGSEWRYVLVSTVRTCAKSDLDQRPTKSWLKKFLGFVVDPNQVNVAVTRAQEGLCLIGDHLLLRCCPLWRSLLDFCEAQQTLVPAGQVRVCRRPTMPS 相同性解析による交差性が予測される生物種 |
| Full Name | peroxisomal proliferator-activated receptor A interacting complex 285 |
| Calculated molecular weight | 2649 aa, 295 kDa |
| Observed molecular weight | 294 kDa |
| GenBank accession number | BC150283 |
| Gene Symbol | PRIC285 |
| Gene ID (NCBI) | 85441 |
| RRID | AB_3742156 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYK8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRIC285, also known as HELZ2, is a member of the E3 ubiquitin ligase family, specifically the RING finger protein family. Its principal function is to serve as a nuclear transcriptional co-regulator within the peroxisome proliferator-activated receptor (PPAR) signaling pathway, thereby influencing lipid metabolism and energy homeostasis through the regulation of gene expression.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PRIC285 antibody 25736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |