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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PREPL Polyclonal antibody (12478-1-AP)

    WB analysis of mouse brain using 12478-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PREPL Polyclonal antibody (12478-1-AP)

    WB analysis of human brain using 12478-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PREPL Polyclonal antibody (12478-1-AP)

    IHC staining of mouse brain using 12478-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PREPL Polyclonal antibody (12478-1-AP)

    IHC staining of mouse brain using 12478-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using PREPL Polyclonal antibody (12478-1-AP)

    IHC staining of rat brain using 12478-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using PREPL Polyclonal antibody (12478-1-AP)

    IHC staining of rat brain using 12478-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PREPL Polyclonal antibody

PREPL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12478-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:3.4.21.-, KIAA0436, PPCEL, prolyl endopeptidase like, Prolyl endopeptidase-like

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12478-1-AP targets PREPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3196

Product name: Recombinant human PREPL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-244 aa of BC013193

Sequence: MDLKMNFRPERRVLVDDGWILAYCHVRYGGELGLQWHADGRLTKKLNGLADLEACIKTLHGQGFSQPSLTTLTAFSAGGVLAGALCNSNPELVRAVTLEAPFLDVLNTMMDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPYQNIKPQHYPSIHITAYENDERVPLKGIVSYTEKLKEAIAEHAKDTGEGYQTPNIILDIQPGGNHVIEDSHKKITAQIKFLYEELGLDSTSVFEDLKKYLKF

相同性解析による交差性が予測される生物種
Full Name prolyl endopeptidase-like
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC013193
Gene Symbol PREPL
Gene ID (NCBI) 9581
RRIDAB_2300040
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4J6C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PREPL (Prolyl endopeptidase-like) is also named as KIAA0436 and belongs to the prolyl oligopeptidase subfamily of serine peptidases. IPREPL is a novel oligopeptidase with unique structural and functional characteristics (PMID:16385448). It has 4 isoforms produced by alternative splicing and can exsit as a homodimer (PMID:16143824). Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS).

Protocols

Product Specific Protocols
IHC protocol for PREPL antibody 12478-1-APDownload protocol
WB protocol for PREPL antibody 12478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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