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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRELP Polyclonal antibody (23783-1-AP)

    WB analysis using 23783-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23783-1-AP (PRELP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRELP Polyclonal antibody (23783-1-AP)

    IHC staining of human stomach cancer using 23783-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23783-1-AP (PRELP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRELP Polyclonal antibody (23783-1-AP)

    IHC staining of human stomach cancer using 23783-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23783-1-AP (PRELP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PRELP Polyclonal antibody (23783-1-AP)

    IF Staining of U2OS using 23783-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PRELP antibody (23783-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

PRELP Polyclonal antibody

PRELP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23783-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

SLRR2A, Proline-arginine-rich end leucine-rich repeat protein, Prolargin, MSTP161, MST161

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, SMMC-7721 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23783-1-AP targets PRELP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRELP fusion protein Ag20656 相同性解析による交差性が予測される生物種
Full Name proline/arginine-rich end leucine-rich repeat protein
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC032498
Gene Symbol PRELP
Gene ID (NCBI) 5549
RRIDAB_3085714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRELP (proline/arginine-rich end leucine-rich repeat protein), also known as MST161, MSTP161, prolargin, or SLRR2A, belongs to the class II subfamily of the small leucine-rich proteoglycan (SLRP) family. PRELP is a small leucine-rich proteoglycan (SLRP), which binds collagen to basement membranes or cartilage as a matrix protein. PRELP has prognostic value and regulates cell proliferation and migration in hepatocellular carcinoma, may serve as a potential prognostic marker and a treatment target against HCC progression (PMID: 33033521).

Protocols

Product Specific Protocols
WB protocol for PRELP antibody 23783-1-APDownload protocol
IHC protocol for PRELP antibody 23783-1-APDownload protocol
IF protocol for PRELP antibody 23783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,CoIP

Apoptosis

PRELP inhibits colorectal cancer progression by suppressing epithelial-mesenchymal transition and angiogenesis via the inactivation of the FGF1/PI3K/AKT pathway

Authors - Xiaoqing Li
  • KD Validated
View Article
humanWB,IHC

Front Med (Lausanne)

Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma

Authors - Xiaoxia Pan
View Article