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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRELP Polyclonal antibody

PRELP Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 23783-1-AP

Synonyms

MST161, MSTP161, PRELP, Prolargin, SLRR2A

Validation Data Gallery

Applications:
  • WB analysis using 23783-1-AP

    WB analysis using 23783-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23783-1-AP (PRELP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 23783-1-AP

    IF Staining of U2OS using 23783-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PRELP antibody (23783-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, SMMC-7721 cells
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

23783-1-AP targets PRELP in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRELP fusion protein Ag20656 相同性解析による交差性が予測される生物種
Full Name proline/arginine-rich end leucine-rich repeat protein
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC032498
Gene symbol PRELP
Gene ID (NCBI) 5549
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRELP (proline/arginine-rich end leucine-rich repeat protein), also known as MST161, MSTP161, prolargin, or SLRR2A, belongs to the class II subfamily of the small leucine-rich proteoglycan (SLRP) family. PRELP is a small leucine-rich proteoglycan (SLRP), which binds collagen to basement membranes or cartilage as a matrix protein. PRELP has prognostic value and regulates cell proliferation and migration in hepatocellular carcinoma, may serve as a potential prognostic marker and a treatment target against HCC progression (PMID: 33033521).

Protocols

Product Specific Protocols
WB protocol for PRELP antibody 23783-1-APDownload protocol
IF protocol for PRELP antibody 23783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols