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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRDX1 Monoclonal antibody (66820-1-Ig)

    WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PRDX1 Monoclonal antibody (66820-1-Ig)

    WB analysis of HEK-293 using 66820-1-Ig (same clone as 66820-1-PBS)

    WB result of PRDX1 antibody (66820-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using PRDX1 Monoclonal antibody (66820-1-Ig)

    WB analysis of U2OS using 66820-1-Ig (same clone as 66820-1-PBS)

    U2OS (WT and PRDX1 KO) lysates prepared with RIPA buffer, 10 μg protein loaded. 66820-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRDX1 Monoclonal antibody (66820-1-Ig)

    WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRDX1 Monoclonal antibody (66820-1-Ig)

    IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRDX1 Monoclonal antibody (66820-1-Ig)

    IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using PRDX1 Monoclonal antibody (66820-1-Ig)

    IF Staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PRDX1 antibody (66820-1-Ig, Clone: 2B2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PRDX1 Monoclonal antibody, PBS Only

PRDX1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66820-1-PBS
Clone No.2B2A2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

2B2A2, EC:1.11.1.24, MSP23, Natural killer cell-enhancing factor A, NKEF-A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66820-1-PBS targets PRDX1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8821

Product name: Recombinant human PRDX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-199 aa of BC007063

Sequence: MSSGNAKIGHPAPNFKATAVMPDGQFKDISLSDYKGKYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIGASVDSHFCHLAWVNTPKKQGGLGPMNIPLVSDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITVNDLPVGRSVDETLRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVQKSKEYFSKQK

相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 1
Calculated molecular weight 199 aa, 22 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC007063
Gene Symbol PRDX1
Gene ID (NCBI) 5052
RRIDAB_2882163
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ06830
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).