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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50344-1

    Sandwich ELISA standard curve of MP50344-1

    Sandwich ELISA standard curve of MP50344-1, Human PRAME Monoclonal Matched Antibody Pair - PBS only. 68097-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1906. 68097-3-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

  • Sample test of MP50344-1

    Sample test of MP50344-1

    The mean PRAME concentration was determined to be 230.66 ng/mL in K-562 cell extract based on a 2.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP50344-1

    Cytometric bead array standard curve of MP50344-1

    Cytometric bead array standard curve of MP50344-1, PRAME Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68097-2-PBS. Detection antibody: 68097-3-PBS. Standard:Ag1906. Range: 6.25-100 ng/mL.

PRAME Monoclonal antibody, PBS Only (Capture)

PRAME Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68097-2-PBS
Clone No.5H11B6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Opa-interacting protein 4, OIP-4, OIP4, OIP 4, MAPE

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68097-2-PBS targets PRAME as part of a matched antibody pair:

MP50344-1: 68097-2-PBS capture and 68097-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1906

Product name: Recombinant human PRAME protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-509 aa of BC014074

Sequence: MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMPN

相同性解析による交差性が予測される生物種
Full Name preferentially expressed antigen in melanoma
Calculated molecular weight 509 aa, 58 kDa
GenBank accession numberBC014074
Gene Symbol PRAME
Gene ID (NCBI) 23532
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP78395
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.