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Validation Data Gallery

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  • Western Blot (WB) analysis of Sp2/0 cells using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of Sp2/0 using 17106-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Sp2/0 cells using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of Sp2/0 using 17106-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of mouse brain using 17106-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of mouse kidney using 17106-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of mouse testis using 17106-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of HeLa using 17106-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of mouse liver using 17106-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PPWD1 Polyclonal antibody (17106-1-AP)

    WB analysis of mouse liver using 17106-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PPWD1 Polyclonal antibody

PPWD1 Polyclonal Antibody for WB, ELISA
Cat No. 17106-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA0073, PPWD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inSp2/0 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17106-1-AP targets PPWD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10785

Product name: Recombinant human PPWD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 296-646 aa of BC015385

Sequence: ATIGSDRKVRIFRFVTGKLMRVFDESLSMFTELQQMRQQLPDMEFGRRMAVERELEKVDAVRLINIVFDETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVMQLALFQGIAKKHRAATTIEMKASENPVLQNIQADPTIVCTSFKKNRFYMFTKREPEDTKSADSDRDVFNEKPSKEEVMAATQAEGPKRVSDSAIIHTSMGDIHTKLFPVECPKTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPYTLSMANAGSNTNGSQFFITVVPTPWLDNKHTVFGRVTKGMEVVQRISNVKVNPKTDKPYEDVSIINITVK

相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase domain and WD repeat containing 1
Calculated molecular weight 646 aa, 74 kDa
Observed molecular weight 65-66 kDa
GenBank accession numberBC015385
Gene Symbol PPWD1
Gene ID (NCBI) 23398
RRIDAB_2237509
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peptidylprolyl isomerase domain and WD repeat-containing protein1 belongs to cyclophilin family.PPWD1 accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.This antibody recognizes the C-terminal of PPWD1.

Protocols

Product Specific Protocols
WB protocol for PPWD1 antibody 17106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

A roadmap for ribosome assembly in human mitochondria

Authors - Elena Lavdovskaia
View Article