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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPT1 Polyclonal antibody (29653-1-AP)

    WB analysis using 29653-1-AP (same clone as 29653-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29653-1-AP (PPT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PPT1 Polyclonal antibody (29653-1-AP)

    WB analysis of A549 using 29653-1-AP (same clone as 29653-1-PBS)

    WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells. This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPT1 Polyclonal antibody (29653-1-AP)

    IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPT1 Polyclonal antibody (29653-1-AP)

    IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 10x lens). Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PPT1 Polyclonal antibody (29653-1-AP)

    IF Staining of mouse testis using 29653-1-AP (same clone as 29653-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using PPT1 Polyclonal antibody (29653-1-AP)

    IF Staining of rat brain using 29653-1-AP (same clone as 29653-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PPT1 Polyclonal antibody, PBS Only

PPT1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 29653-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CLN1, EC:3.1.2.22, INCL, Palmitoyl-protein hydrolase 1, Palmitoyl-protein thioesterase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29653-1-PBS targets PPT1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31240

Product name: Recombinant human PPT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 150-306 aa of BC008426

Sequence: PRCPGESSHICDFIRKTLNAGAYSKVVQERLVQAEYWHDPIKEDVYRNHSIFLADINQERGINESYKKNLMALKKFVMVKFLNDSIVDPVDSEWFGFYRSGQAKETIPLQETSLYTQDRLGLKEMDNAGQLVFLATEGDHLQLSEEWFYAHIIPFLG

相同性解析による交差性が予測される生物種
Full Name palmitoyl-protein thioesterase 1
Calculated molecular weight 34 kDa
Observed molecular weight32-34 kDa
GenBank accession numberBC008426
Gene Symbol PPT1
Gene ID (NCBI) 5538
RRIDAB_2923599
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP50897
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

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