Validation Data Gallery
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Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, MDA-MB-453 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32194-1-AP targets PPPDE2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPPDE2 fusion protein Ag37655 相同性解析による交差性が予測される生物種 |
| Full Name | PPPDE peptidase domain containing 2 |
| Calculated molecular weight | 168 aa, 18 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC112179 |
| Gene Symbol | PPPDE2 |
| Gene ID (NCBI) | 27351 |
| RRID | AB_3670220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6ICB0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPPDE2 belongs to N1pC/P60 superfamily of papain-like enzymes, which comprise an extremely diverse group of proteins originating from archaea, bacteria, bacteriophages, viruses, and eukaryotes with predicted protease, amidase, transglutaminases, or acyltransferase activity but often uncharacterized function (PMID: 12620121). PPPDE2 exhibits palmitoyl protein thioesterase (S-depalmitoylation) activity towards synthetic substrates 4-methylumbelliferyl-6-S-palmitoyl-beta-D-glucopyranoside and S-depalmitoylation probe 5 (DPP-5) (PMID:35427157).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPPDE2 antibody 32194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |