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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP6C Recombinant monoclonal antibody (87155-1-RR)

    WB analysis using 87155-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87155-1-RR (PPP6C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PPP6C Recombinant monoclonal antibody (87155-1-RR)

    IF Staining of HEK-293 using 87155-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PPP6C antibody (87155-1-RR, Clone: 252251E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87155-1-RR

    Affinity of 87155-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87155-1-RR against Human PPP6C were performed. The affinity constant is 49.7 nM.

PPP6C Recombinant monoclonal antibody

PPP6C Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87155-1-RR
Clone No.252251E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:3.1.3.16, PP6C, PPP6, Serine/threonine-protein phosphatase 6 catalytic subunit, Serine/threonine-protein phosphatase 6 catalytic subunit, N-terminally processed

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87155-1-RR targets PPP6C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8554

Product name: Recombinant human PPP6C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-305 aa of BC006990

Sequence: MAPLDLDKYVEIARLCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQVPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWRYCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPRGAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTREPKLFRAVPDSERVIPPRTTTPYFL

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 6, catalytic subunit
Calculated molecular weight 305 aa, 35 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC006990
Gene Symbol PPP6C
Gene ID (NCBI) 5537
RRIDAB_3745376
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00743
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP6C (Protein Phosphatase 6 Catalytic Subunit or simply PP6) is a highly conserved serine/threonine phosphatase belonging to the PP2A-like subfamily. This enzyme complex plays critical roles in a wide array of fundamental cellular processes, including cell cycle progression, DNA damage response, NF-κB signaling, and microtubule organization. PPP6C mutations are found across multiple cancer types but are most common in melanoma and other skin cancers (PMID: 33789117).

Protocols

Product Specific Protocols
IF protocol for PPP6C antibody 87155-1-RRDownload protocol
WB protocol for PPP6C antibody 87155-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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