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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP4R1 Monoclonal antibody (67392-1-Ig)

    WB analysis using 67392-1-Ig (same clone as 67392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67392-1-Ig (PPP4R1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R1 Monoclonal antibody (67392-1-Ig)

    IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R1 Monoclonal antibody (67392-1-Ig)

    IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using PPP4R1 Monoclonal antibody (67392-1-Ig)

    IF Staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using PPP4R1 Monoclonal antibody (67392-1-Ig)

    IF Staining of mouse liver using 67392-1-Ig (same clone as 67392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PPP4R1 Monoclonal antibody (67392-1-Ig)

    FC experiment of HeLa using 67392-1-Ig (same clone as 67392-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPP4R1 (67392-1-Ig, Clone:3D12G12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

PPP4R1 Monoclonal antibody, PBS Only

PPP4R1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67392-1-PBS
Clone No.3D12G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

3D12G12, MEG1, PP4(Rmeg), PP4R1, Serine/threonine-protein phosphatase 4 regulatory subunit 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67392-1-PBS targets PPP4R1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26750

Product name: Recombinant human PPP4R1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 331-520 aa of BC060829

Sequence: NRTRDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKELEEMIENLEPHIDDPDVKAQVEVLSAALRAS

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 4, regulatory subunit 1
Calculated molecular weight 107 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC060829
Gene Symbol PPP4R1
Gene ID (NCBI) 9989
RRIDAB_2882636
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8TF05
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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