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Validation Data Gallery

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  • Western Blot (WB) analysis of human ileum tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis of human ileum using 12675-2-AP (same clone as 12675-2-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)

    WB result of PPP2R5A antibody (12675-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP2R5A transfected HeLa cells. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis using 12675-2-AP (same clone as 12675-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis of human brain using 12675-2-AP (same clone as 12675-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis of human heart using 12675-2-AP (same clone as 12675-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis of human kidney using 12675-2-AP (same clone as 12675-2-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    WB analysis of human lung using 12675-2-AP (same clone as 12675-2-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    IP experiment of mouse brain using 12675-2-AP (same clone as 12675-2-PBS)

    IP result of anti-PPP2R5A (IP:12675-2-AP, 4ug; Detection:12675-2-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PPP2R5A Polyclonal antibody (12675-2-AP)

    IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PPP2R5A Polyclonal antibody (12675-2-AP)

    IF Staining of PC-3 using 12675-2-AP (same clone as 12675-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PPP2R5A antibody (12675-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PPP2R5A Polyclonal antibody, PBS Only

PPP2R5A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12675-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

B56A, PP2A B subunit isoform B alpha, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform PR61-alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12675-2-PBS targets PPP2R5A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3360

Product name: Recombinant human PPP2R5A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 188-486 aa of BC022474

Sequence: FVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENINKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2, regulatory subunit B', alpha isoform
Calculated molecular weight 486 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC022474
Gene Symbol PPP2R5A
Gene ID (NCBI) 5525
RRIDAB_2252956
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15172
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.

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