Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, MCF-7 cells |
| Positive IHC detected in | human skin tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
14720-1-AP targets PPP2R3A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPP2R3A fusion protein Ag6450 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha |
| Calculated molecular weight | 130 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC065531 |
| Gene Symbol | PPP2R3A |
| Gene ID (NCBI) | 5523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06190 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPP2R3A(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha), also named as PPP2R3, has 2 isoforms produced by alternative splicing named as PR130 and PR72. The protein contains stretches of basic residues that may be putative nuclear localization signals, and a cluster of proline residues in an N-terminal region common to both isoforms that the isoform PR130 but not PR72 is variably expressed in a number of tumor cell lines. The PR72 and PR130 cDNAs encode 72 and 130 kDa proteins when expressed in rabbit reticulocyte lysates.(PMID:8392071).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R3A antibody 14720-1-AP | Download protocol |
| IHC protocol for PPP2R3A antibody 14720-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Biosci GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis. | ||
Cell Signal PP2A-AMPKα-HSF1 axis regulates metal-induced HSP expression and ROS clearance. |