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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    WB analysis of MCF-7 using 13123-1-AP (same clone as 13123-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    IP experiment of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

    IP result of anti-PPP2R2B/A/C/D (IP:13123-1-AP, 4ug; Detection:13123-1-AP 1:500) with SH-SY5Y cells lysate 1200ug. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    IHC staining of mouse brain using 13123-1-AP (same clone as 13123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PPP2R2B/A/C/D Polyclonal antibody (13123-1-AP)

    IF Staining of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2B/A/C/D antibody (13123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

PPP2R2B/A/C/D Polyclonal antibody, PBS Only

PPP2R2B/A/C/D Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13123-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13123-1-PBS targets PPP2R2B/A/C/D in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3860

Product name: Recombinant human PPP2R2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-443 aa of BC031790

Sequence: QQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQDKVN

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
Calculated molecular weight 443 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC031790
Gene Symbol PPP2R2B
Gene ID (NCBI) 5521
RRIDAB_2169964
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00005
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.

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