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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2R2A Polyclonal antibody (16569-1-AP)

    WB analysis using 16569-1-AP (same clone as 16569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16569-1-AP (PPP2R2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2R2A Polyclonal antibody (16569-1-AP)

    WB analysis using 16569-1-AP (same clone as 16569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16569-1-AP (PPP2R2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PPP2R2A Polyclonal antibody (16569-1-AP)

    IHC staining of mouse brain using 16569-1-AP (same clone as 16569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PPP2R2A Polyclonal antibody (16569-1-AP)

    IHC staining of mouse brain using 16569-1-AP (same clone as 16569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PPP2R2A Polyclonal antibody (16569-1-AP)

    IF Staining of A431 using 16569-1-AP (same clone as 16569-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPP2R2A antibody (16569-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

PPP2R2A Polyclonal antibody, PBS Only

PPP2R2A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16569-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16569-1-PBS targets PPP2R2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9851

Product name: Recombinant human PPP2R2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 99-447 aa of BC041071

Sequence: IRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha isoform
Calculated molecular weight 477 aa, 52 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC041071
Gene Symbol PPP2R2A
Gene ID (NCBI) 5520
RRIDAB_2878280
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP63151
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The serine/threonine protein phosphatase 2A (PP2A) is a master regulator of the complex cellular signaling that occurs during all stages of mammalian development. PPP2R2A, also known as B55A or PR55A, is a regulatory subunit of PP2A expressed in an Akt-dependent manner in epidermis during barrier formation. PPP2R2A participates in the negative control of cell growth and division. PPP2R2A has been proved to be a tumor suppressor that can inhibit the proliferation of a variety of cancer cells, such as prostate cancer cells(PMID: 21872824).

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