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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2CA Monoclonal antibody (67809-1-Ig)

    WB analysis using 67809-1-Ig (same clone as 67809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67809-1-Ig (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Monoclonal antibody (67809-1-Ig)

    IHC staining of human liver using 67809-1-Ig (same clone as 67809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Monoclonal antibody (67809-1-Ig)

    IHC staining of human liver using 67809-1-Ig (same clone as 67809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PPP2CA Monoclonal antibody, PBS Only (67809-1-PBS)

    FC experiment of MCF-7 using 67809-1-PBS

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PPP2CA Monoclonal antibody, PBS Only (67809-1-PBS, Clone:3A6H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

PPP2CA Monoclonal antibody, PBS Only

PPP2CA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67809-1-PBS
Clone No.3A6H5

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

3A6H5, EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67809-1-PBS targets PPP2CA in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4294

Product name: Recombinant human PPP2CA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-309 aa of BC031696

Sequence: MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight 309 aa, 36 kDa
Observed molecular weight33 kDa
GenBank accession numberBC031696
Gene Symbol PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2918572
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP67775
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Protocols

Product Specific Protocols
FC protocol for PPP2CA antibody 67809-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols