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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using PPP1R8 Polyclonal antibody (16115-1-AP)

    WB analysis of PC-3 using 16115-1-AP (same clone as 16115-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PPP1R8 Polyclonal antibody (16115-1-AP)

    WB analysis of HepG2 using 16115-1-AP (same clone as 16115-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human kidney using 16115-1-AP (same clone as 16115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human kidney using 16115-1-AP (same clone as 16115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human testis using 16115-1-AP (same clone as 16115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human testis using 16115-1-AP (same clone as 16115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human spleen using 16115-1-AP (same clone as 16115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human spleen using 16115-1-AP (same clone as 16115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R8 Polyclonal antibody (16115-1-AP)

    IF Staining of HepG2 using 16115-1-AP (same clone as 16115-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

PPP1R8 Polyclonal antibody, PBS Only

PPP1R8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16115-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16115-1-PBS targets PPP1R8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9041

Product name: Recombinant human PPP1R8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-209 aa of BC013360

Sequence: MGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 8
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 47 kDa, 28 kDa, 19 kDa
GenBank accession numberBC013360
Gene Symbol PPP1R8
Gene ID (NCBI) 5511
RRIDAB_2169304
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12972
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.

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