Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55389-1-AP targets PPP1R3G in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 3G |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 38-40 kDa |
| GenBank accession number | NM_001145115 |
| Gene Symbol | PPP1R3G |
| Gene ID (NCBI) | 648791 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | B7ZBB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPP1R3G (protein phosphatase 1 regulatory subunit 3G) is an important member of phosphatase 1 inhibitor -3 family, which is mainly expressed in liver and muscle and participates in glycogen synthesis and metabolism, insulin signaling pathway and lipid metabolism. PPP1R3G is the direct target of insulin -AKT signaling pathway. Insulin phosphorylates Ser79 site of PPP1R3G through AKT, which promotes its binding with GS, so that GS is dephosphorylated by PP1, thus enhancing glycogen synthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R3G antibody 55389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |