Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, mouse cerebellum tissue, rat cerebellum tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
29424-1-AP targets PPP1R3C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30280 Product name: Recombinant human PPP1R3C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 42-185 aa of BC012625 Sequence: PYDEFQRRHFVNKLKPLKSCLNIKHKAKSQNDWKCSHNQAKKRVVFADSKGLSLTAIHVFSDLPEEPAWDLQFDLLDLNDISSALKHHEEKNLILDFPQPSTDYLSFRSHFQKNFVCLENCSLQERTVTGTVKVKNVSFEKKVQ 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 3C |
| Calculated molecular weight | 317 aa, 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC012625 |
| Gene Symbol | PPP1R3C |
| Gene ID (NCBI) | 5507 |
| RRID | AB_2918304 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQK1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
• Protein phosphatase 1 regulatory subunit 3C (PPP1R3C) is an enzyme that binds to protein phosphatase-1 (PP1) as a regulator. The central role of PPP1R3C is associated with glycogenesis and the overexpression of this gene increases glycogen storage. The cellular glycogen level is suppressed when PPP1R3C is knocked-down. PPP1R3C induces glycogen accumulation via HIF1 α during hypoxia and it is hypermethylated in melanoma (PMID: 25846879). Overexpression of PPP1R3C increased hepatic gluconeogenesis in vitro and in vivo while knockdown of PPP1R3C showed an opposite result (PMID: 31181215).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP1R3C antibody 29424-1-AP | Download protocol |
| WB protocol for PPP1R3C antibody 29424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |