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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R3A Polyclonal antibody (22495-1-AP)

    WB analysis using 22495-1-AP (same clone as 22495-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22495-1-AP (PPP1R3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22495-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using PPP1R3A Polyclonal antibody (22495-1-AP)

    IF Staining of THP-1 using 22495-1-AP (same clone as 22495-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PPP1R3A antibody (22495-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22495-1-PBS in a different storage buffer formulation.

PPP1R3A Polyclonal antibody, PBS Only

PPP1R3A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 22495-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RG1, Protein phosphatase type-1 glycogen targeting subunit, Protein phosphatase 1 glycogen-associated regulatory subunit, PPP1R3, PP1G

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22495-1-PBS targets PPP1R3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18013

Product name: Recombinant human PPP1R3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 773-1122 aa of BC126453

Sequence: FDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDHRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 3A
Calculated molecular weight 1122 aa, 126 kDa
Observed molecular weight110-130 kDa
GenBank accession numberBC126453
Gene Symbol PPP1R3A
Gene ID (NCBI) 5506
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ16821
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPP1R3A (Protein Phosphatase 1 Regulatory Subunit 3A), also known as Protein Targeting to Glycogen (PTG), is a regulatory subunit of protein phosphatase 1 (PP1), a major serine/threonine phosphatase involved in metabolic regulation. It plays a crucial role in glycogen metabolism by targeting PP1 to glycogen particles, facilitating glycogen synthesis and breakdown. PPP1R3A is involved in muscle glycogen regulation (PMID: 24264575).

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