Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12461-1-AP targets PPP1R1A in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3128 Product name: Recombinant human PPP1R1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC022470 Sequence: MEQDNSPQKIQFTVPLLEPHLDPEAAEQIRRRRPTPATLVLTSDQSSPEIDEDRIPNPHLKSTLAMSPRQRKKMTRITPTMKELQMMVEHHLGQQQQGEEPEGAAESTGTQESRPPGIPDTEVESRLGTSGTAKKTAECIPKTHERGSKEPSTKEPSTHIPPLDSKGANSV 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 1A |
| Calculated molecular weight | 171 aa, 19 kDa |
| Observed molecular weight | 15-18 kDa |
| GenBank accession number | BC022470 |
| Gene Symbol | PPP1R1A |
| Gene ID (NCBI) | 5502 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13522 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPP1R1A, also known as protein phosphatase 1 regulatory subunit 1A, is a gene that plays a crucial role in regulating the activity of protein phosphatase 1 (PP1). As an inhibitor, PPP1R1A binds to PP1, preventing the dephosphorylation of PP1 to other proteins. This gene influences a broad range of cellular functions by maintaining the phosphorylation of specific proteins and is directly involved in glycogen metabolism, cardiac contractility, and cell proliferation. PPP1R1A is considered a key factor in regulating cellular signaling and physiological homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R1A antibody 12461-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |