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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R1A Polyclonal antibody (12461-1-AP)

    WB analysis using 12461-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12461-1-AP (PPP1R1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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PPP1R1A Polyclonal antibody

PPP1R1A Polyclonal Antibody for WB, ELISA
Cat No. 12461-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

IPP-1, IPP1, IPP 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12461-1-AP targets PPP1R1A in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R1A fusion protein Ag3128 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 1A
Calculated molecular weight 171 aa, 19 kDa
Observed molecular weight15-18 kDa
GenBank accession numberBC022470
Gene Symbol PPP1R1A
Gene ID (NCBI) 5502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13522
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R1A, also known as protein phosphatase 1 regulatory subunit 1A, is a gene that plays a crucial role in regulating the activity of protein phosphatase 1 (PP1). As an inhibitor, PPP1R1A binds to PP1, preventing the dephosphorylation of PP1 to other proteins. This gene influences a broad range of cellular functions by maintaining the phosphorylation of specific proteins and is directly involved in glycogen metabolism, cardiac contractility, and cell proliferation. PPP1R1A is considered a key factor in regulating cellular signaling and physiological homeostasis.

Protocols

Product Specific Protocols
WB protocol for PPP1R1A antibody 12461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols