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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R17 Polyclonal antibody (27976-1-AP)

    WB analysis using 27976-1-AP (same clone as 27976-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27976-1-AP (C7orf16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PPP1R17 Polyclonal antibody (27976-1-AP)

    IHC staining of mouse cerebellum using 27976-1-AP (same clone as 27976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27976-1-AP (PPP1R17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPP1R17 Polyclonal antibody (27976-1-AP)

    IF Staining of U2OS using 27976-1-AP (same clone as 27976-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPP1R17 Polyclonal antibody (27976-1-AP)

    IF Staining of U2OS using 27976-1-AP (same clone as 27976-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

PPP1R17 Polyclonal antibody, PBS Only

PPP1R17 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27976-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C7orf16, G substrate, GSBS, G-substrate, Protein phosphatase 1 regulatory subunit 17

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27976-1-PBS targets PPP1R17 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15187

Product name: Recombinant human C7orf16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-155 aa of BC028094

Sequence: MMSTEQMQPLEVSEDRLDKLDPRCSHLDDLSDQFIKDCDLKKKPRKGKNVQATLNVESDQKKPRRKDTPALHIPPFIPGVFSEHLIKRYDVQERHPKGKMIPVLHNTDLEQKKPRRKDTPALHMSPFAAGVTLLRDERPKAIVEDDEKDGDKIAI

相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 16
Calculated molecular weight 155 aa, 18 kDa
Observed molecular weight 20-30 kDa
GenBank accession numberBC028094
Gene Symbol PPP1R17
Gene ID (NCBI) 10842
RRIDAB_3086018
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO96001
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPP1R17, an endogenous substrate for cGMP-dependent protein kinase, exists almost exclusively in cerebellar Purkinje cells, where it is possibly involved in the induction of long-term depression (PMID: 10051666). PPP1R17 inhibits phosphatase activities of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) complexes. Allelic variants increase susceptibility to hypercholesterolemia (PMID: 12955585).

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