Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25357-1-AP targets PPP1R16B in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19269 Product name: Recombinant human PPP1R16B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 351-565 aa of BC131801 Sequence: LSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCR 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 16B |
| Calculated molecular weight | 567 aa, 64 kDa |
| Observed molecular weight | 60 kDa, 70 kDa |
| GenBank accession number | BC131801 |
| Gene Symbol | PPP1R16B |
| Gene ID (NCBI) | 26051 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96T49 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPP1R16B (Protein Phosphatase 1 Regulatory Subunit 16B), also known as TIMAP or ANKRD4, is a membrane-associated protein that acts as a regulator of protein phosphatase 1 (PP1). It contains five ankyrin repeats, a PP1-interacting domain, and a C-terminal CAAX box domain. The synthesis of PPP1R16B is inhibited by transforming growth factor-beta-1 (TGF-β-1), and it is predominantly expressed in endothelial cells. It has 2 isoforms with the molecular mass of 59 and 64 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R16B antibody 25357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |