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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP1R14B Polyclonal antibody

PPP1R14B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18476-1-AP

Synonyms

PHI 1, PLCB3N, PNG, PPP1R14B, SOM172

Validation Data Gallery

Applications:
  • WB analysis using 18476-1-AP

    WB analysis using 18476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 18476-1-AP

    WB analysis of MCF-7 using 18476-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 18476-1-AP

    WB analysis of U2OS using 18476-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 18476-1-AP

    WB analysis of A549 using 18476-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 18476-1-AP

    WB analysis of mouse liver using 18476-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 18476-1-AP

    WB analysis of human heart using 18476-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 18476-1-AP

    WB analysis of mouse skeletal muscle using 18476-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, mouse liver tissue, human heart tissue, mouse skeletal muscle tissue, U2OS cells, A549 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

18476-1-AP targets PPP1R14B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R14B fusion protein Ag13373 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14B
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC014522
Gene symbol PPP1R14B
Gene ID (NCBI) 26472
RRIDAB_10598019
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP1R14B antibody 18476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Immunity

Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis.

Authors - Kiyoharu Fukushima
View Article
humanWB,IHC

J Cell Mol Med

PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma

Authors - Kang He
View Article