Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87808-1-RR targets PPP1R12B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4185 Product name: Recombinant human PPP1R12B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-386 aa of BC034430 Sequence: MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEAVLFWPF 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 12B |
| Calculated molecular weight | 982 aa, 110 kDa |
| Observed molecular weight | 110-130 kDa |
| GenBank accession number | BC034430 |
| Gene Symbol | PPP1R12B |
| Gene ID (NCBI) | 4660 |
| RRID | AB_3745761 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPP1R12B (MYPT2) is a critical regulatory subunit of protein phosphatase 1, primarily functioning in muscle tissues to control myosin phosphatase activity and calcium sensitivity of contraction. Its tissue-specific expression, particularly enrichment in heart and skeletal muscle, along with multiple alternatively spliced isoforms, enables specialized roles in different cellular contexts. The protein's ankyrin repeats and C-terminal kinase-interacting domain facilitate its function as a molecular scaffold that directs PP1 catalytic activity toward specific substrates.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP1R12B antibody 87808-1-RR | Download protocol |
| WB protocol for PPP1R12B antibody 87808-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |