Validation Data Gallery

Filter: AllWBIHC

WB analysis using 14435-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14435-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14435-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14435-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14435-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 14435-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 14435-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14435-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14435-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPME1 Polyclonal antibody

PPME1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14435-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:3.1.1.89, PME 1, PME1, PME-1, PP2593

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected in	mouse brain tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:4000-1:20000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

14435-1-AP targets PPME1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPME1 fusion protein Ag5817 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase methylesterase 1
Calculated molecular weight	42 kDa
Observed molecular weight	44 kDa
GenBank accession number	BC050705
Gene Symbol	PPME1
Gene ID (NCBI)	51400
RRID	AB_2284364
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y570
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291).

Protocols

Product Specific Protocols
WB protocol for PPME1 antibody 14435-1-AP	Download protocol
IHC protocol for PPME1 antibody 14435-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Immunol CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses. Authors - Xiaowen Bi View Article
human	WB	Neural Regen Res FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression Authors - Jing Yao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14435-1-AP

WB analysis of A549 using 14435-1-AP

WB analysis of HeLa using 14435-1-AP

WB analysis of mouse brain using 14435-1-AP

WB analysis of human brain using 14435-1-AP

WB analysis of fetal human brain using 14435-1-AP

IHC staining of mouse brain using 14435-1-AP

IHC staining of mouse testis using 14435-1-AP