Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16269-1-AP targets PPM1J in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9336 Product name: Recombinant human PPM1J protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-488 aa of BC011803 Sequence: DSRAIIVRNGEIIPMSREFTPETERQRLQLLGFLKPELLGSEFTHLEFPRRVLPKELGQRMLYRDQNMTGWAYKKIELEDLRFPLVCGEGKKARVMATIGVTRGLGDHSLKVCSSTLPIKPFLSCFPEVRVYDLTQYEHCPDDVLVLGTDGLWDVTTDCEVAATVDRVLSAYEPNDHSRYTALAQALVLGARGTPRDRGWRLPNNKLGSGDDISVFVIPLGGPGSYS 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1J (PP2C domain containing) |
| Calculated molecular weight | 505 aa, 54 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC011803 |
| Gene Symbol | PPM1J |
| Gene ID (NCBI) | 333926 |
| RRID | AB_3669235 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JR12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPM1J also known as PPP2CZ, PP2C-zeta, belongs to the PP2C family. It has a unique N-terminal region and a proline-rich domain between conserved motifs II and III.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPM1J antibody 16269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |