Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13767-1-AP targets PPM1H in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4750 Product name: Recombinant human PPM1H protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-82 aa of BC040177 Sequence: MLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAE 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1H (PP2C domain containing) |
| Calculated molecular weight | 82 aa, 9 kDa |
| Observed molecular weight | 50-56 kDa |
| GenBank accession number | BC040177 |
| Gene Symbol | PPM1H |
| Gene ID (NCBI) | 57460 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPM1H (Protein phosphatase 1H ) is a member of a group of 3 PPM phosphatases that have been termed the 'PPM1H subfamily. In blood cells, PPM1H is expressed at high levels in basophils and lower levels in neutrophils and is very low or not detected in most other cells including monocytes. PPM1H is localized to the Golgi and its knockdown suppresses primary cilia formation, similar to pathogenic LRRK2 (PMID: 27599670, 31663853)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPM1H antibody 13767-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |