• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

PPM1G Polyclonal antibody

PPM1G Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15532-1-AP

Synonyms

PP2C gamma, PP2CG, PP2CGAMMA, PPM1C, PPM1G, PPP2CG, Protein phosphatase 1C, Protein phosphatase 1G

Validation Data Gallery

Applications:
  • WB analysis using 15532-1-AP

    WB analysis using 15532-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 15532-1-AP

    WB analysis of mouse lung using 15532-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15532-1-AP

    IHC staining of mouse testis using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of mouse kidney using 15532-1-AP

    IHC staining of mouse kidney using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of mouse kidney using 15532-1-AP

    IHC staining of mouse kidney using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of mouse testis using 15532-1-AP

    IHC staining of mouse testis using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human placenta using 15532-1-AP

    IHC staining of human placenta using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human placenta using 15532-1-AP

    IHC staining of human placenta using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of mouse spleen using 15532-1-AP

    IHC staining of mouse spleen using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of mouse spleen using 15532-1-AP

    IHC staining of mouse spleen using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human brain using 15532-1-AP

    IHC staining of human brain using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human liver using 15532-1-AP

    IHC staining of human liver using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human liver using 15532-1-AP

    IHC staining of human liver using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human kidney using 15532-1-AP

    IHC staining of human kidney using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human skin using 15532-1-AP

    IHC staining of human skin using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human skin using 15532-1-AP

    IHC staining of human skin using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human brain using 15532-1-AP

    IHC staining of human brain using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human heart using 15532-1-AP

    IHC staining of human heart using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human heart using 15532-1-AP

    IHC staining of human heart using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IHC staining of human kidney using 15532-1-AP

    IHC staining of human kidney using 15532-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PPM1G antibody included
  • IF Staining of MCF-7 using 15532-1-AP

    IF Staining of MCF-7 using 15532-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 15532-1-AP

    IF Staining of MCF-7 using 15532-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293T cells, mouse lung tissue, Jurkat cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue, mouse testis tissue, human heart tissue, human kidney tissue, human skin tissue, human placenta tissue, human brain tissue, mouse spleen tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPM1G Ready-To-Use IHC Kit. PPM1G primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

15532-1-AP targets PPM1G in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPM1G fusion protein Ag7751 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Calculated molecular weight 59 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC000057
Gene symbol PPM1G
Gene ID (NCBI) 5496
RRIDAB_2169934
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

Protocols

Product Specific Protocols
WB protocol for PPM1G antibody 15532-1-APDownload protocol
IHC protocol for PPM1G antibody 15532-1-APDownload protocol
IF protocol for PPM1G antibody 15532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.

Authors - Mao-Xu Ge
View Article
humanWB

J Oncol

Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development

Authors - Weiran Liao
View Article
humanWB,IHC

Cell Death Dis

PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3.

Authors - Dawei Chen
  • KD Validated
View Article