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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPM1E Polyclonal antibody (25089-1-AP)

    WB analysis using 25089-1-AP (same clone as 25089-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25089-1-AP (PPM1E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PPM1E Polyclonal antibody (25089-1-AP)

    IHC staining of rat brain using 25089-1-AP (same clone as 25089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25089-1-AP (PPM1E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PPM1E Polyclonal antibody (25089-1-AP)

    IF Staining of A431 using 25089-1-AP (same clone as 25089-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PPM1E Polyclonal antibody (25089-1-AP)

    IF Staining of SH-SY5Y using 25089-1-AP (same clone as 25089-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

PPM1E Polyclonal antibody, PBS Only

PPM1E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25089-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25089-1-PBS targets PPM1E in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18751

Product name: Recombinant human PPM1E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 501-754 aa of BC136290

Sequence: EESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSY

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1E (PP2C domain containing)
Calculated molecular weight 764 aa, 85 kDa
Observed molecular weight85 kDa
GenBank accession numberBC136290
Gene Symbol PPM1E
Gene ID (NCBI) 22843
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WY54
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N),also known as Ppm1E, is an AMPKα phosphatase,which is up-regulated in human gastric cancer tissues (PMID: 28423719).

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