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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PPM1B Monoclonal antibody (67647-1-Ig)

    IF Staining of A431 using 67647-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPM1B antibody (67647-1-Ig, Clone: 2C7H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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PPM1B Monoclonal antibody

PPM1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67647-1-Ig
Clone No.2C7H2
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2C7H2, EC:3.1.3.16, PP2C beta, PP2CB, PP2C-beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67647-1-Ig targets PPM1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPM1B fusion protein Ag3871 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Calculated molecular weight 479 aa, 53 kDa
Observed molecular weight 36, 53 kDa
GenBank accession numberBC012002
Gene Symbol PPM1B
Gene ID (NCBI) 5495
RRIDAB_2882847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.

Protocols

Product Specific Protocols
WB protocol for PPM1B antibody 67647-1-IgDownload protocol
IHC protocol for PPM1B antibody 67647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

bioRxiv

Epigenetic modulation of NLRP6 inflammasome sensor as a therapeutic modality to reduce necroptosis-driven gastrointestinal mucosal dysfunction in HIV/SIV infection

Authors - Lakmini S Premadasa
View Article