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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of mouse kidney using 12538-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HEK-293 using 12538-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HEK-293 using 12538-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HeLa using 12538-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HEK-293 using 12538-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PPIL4 Polyclonal antibody (12538-1-AP)

    IP experiment of HeLa using 12538-1-AP

    IP result of anti-PPIL4 (IP:12538-1-AP, 4ug; Detection:12538-1-AP 1:300) with HeLa cells lysate 2600 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIL4 Polyclonal antibody (12538-1-AP)

    IF Staining of HeLa using 12538-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPIL4 antibody (12538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PPIL4 Polyclonal antibody

PPIL4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12538-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Cyclophilin like protein PPIL4, Cyclophilin-like protein PPIL4, EC:5.2.1.8, HDCME13P, Peptidyl-prolyl cis-trans isomerase-like 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HCT 116 cells, mouse kidney tissue, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12538-1-AP targets PPIL4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3249

Product name: Recombinant human PPIL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-492 aa of BC016984

Sequence: MDNVLIDDRRIHVDFSQSVAKVKWKGKGGKYTKSDFKEYEKEQDKPPNLVLKDKVKPKQDTKYDLILDEQAEDSKSSHSHTSKKHKKKTHHCSEEKEDEDYMPIKNTNQDIYREMGFGHYEEEESCWEKQKSEKRDRTQNRSRSRSRERDGHYSNSHKSKYQTDLYERERSKKRDRSRSPKKSKDKEKSKYR

相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase (cyclophilin)-like 4
Calculated molecular weight 492 aa, 57 kDa
Observed molecular weight 58-65 kDa
GenBank accession numberBC016984
Gene Symbol PPIL4
Gene ID (NCBI) 85313
RRIDAB_10644441
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peptidyl-prolyl isomerase (PPIase) catalyzes the interconversion of a specific Pro-imide bond between the cis and trans conformations. PPIL4 belongs to the cyclophilin subgroup of the peptidyl-prolyl cis-trans isomerase (PPIase) protein family (PPIs). It contains an RNA recognition motif and a PPIase-like domain. PPIL4, as an IA susceptibility gene, is a novel and key regulatory molecule in Wnt signaling and vertebrate cerebrovascular development (PMID: 34887573).

Protocols

Product Specific Protocols
WB protocol for PPIL4 antibody 12538-1-APDownload protocol
IF protocol for PPIL4 antibody 12538-1-APDownload protocol
IP protocol for PPIL4 antibody 12538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction.

Authors - Wenbo Liu
View Article