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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPIL3 Polyclonal antibody

PPIL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15671-1-AP

Synonyms

Cyclophilin J, Cyclophilin like protein PPIL3, CYPJ, PPIase, PPIL3, Rotamase PPIL3

Validation Data Gallery

Applications:
  • WB analysis using 15671-1-AP

    WB analysis using 15671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15671-1-AP (PPIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15671-1-AP

    IHC staining of human kidney using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIL3 antibody included
  • IHC staining of human kidney using 15671-1-AP

    IHC staining of human kidney using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIL3 antibody included
  • IHC staining of human gliomas using 15671-1-AP

    IHC staining of human gliomas using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIL3 antibody included
  • IHC staining of human gliomas using 15671-1-AP

    IHC staining of human gliomas using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIL3 antibody included

Tested Applications

Positive WB detected inHCT 116 cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPIL3 Ready-To-Use IHC Kit. PPIL3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15671-1-AP targets PPIL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIL3 fusion protein Ag8137 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase (cyclophilin)-like 3
Calculated molecular weight 161 aa, 18 kDa
GenBank accession numberBC007693
Gene symbol PPIL3
Gene ID (NCBI) 53938
RRIDAB_2169615
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPIL3 antibody 15671-1-APDownload protocol
IHC protocol for PPIL3 antibody 15671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols