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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using PPIL2 Polyclonal antibody (12590-2-AP)

    WB analysis of human kidney using 12590-2-AP (same clone as 12590-2-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using PPIL2 Polyclonal antibody (12590-2-AP)

    WB analysis of mouse kidney using 12590-2-AP (same clone as 12590-2-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL2 Polyclonal antibody (12590-2-AP)

    WB analysis of HEK-293 using 12590-2-AP (same clone as 12590-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PPIL2 Polyclonal antibody (12590-2-AP)

    IP experiment of HEK-293 using 12590-2-AP (same clone as 12590-2-PBS)

    IP result of anti-PPIL2 (IP:12590-2-AP, 4ug; Detection:12590-2-AP 1:300) with HEK-293 cells lysate 2600 ug. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

PPIL2 Polyclonal antibody, PBS Only

PPIL2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12590-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Cyclophilin-60, Cyclophilin-like protein Cyp-60, Cyp-60, EC:2.3.2.27, hCyP-60

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12590-2-PBS targets PPIL2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3296

Product name: Recombinant human PPIL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 221-520 aa of BC028385

Sequence: LAATMKAPEKKKVDKLNAAHYSTGKVSASFTSTAMVPETTHEAAAIDEDVLRYQFVKKKGYVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPFKDEFRPNLSHTGRGILSMANSGPNSNRSQFFITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTDRPKEEIRIDATTVFVDPYEEADAQIAQERKTQLKIAPETKVKSSQPQAGSQGPQTFRQGVGKYINPAATKRAAEEEPSTSATVPMSKKKPSRGFGDFSSW

相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase (cyclophilin)-like 2
Calculated molecular weight 520 aa, 59 kDa
Observed molecular weight60~70 kDa
GenBank accession numberBC028385
Gene Symbol PPIL2
Gene ID (NCBI) 23759
RRIDAB_2169606
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13356
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), also known as Cyclophilin-like protein Cyp-60, is a U-box-type E3 ubiquitin ligase belonging to the cyclophilin family. PPIL2 is also a target of the JAK2/STAT5 pathway and upregulated in patients with MPNs (PMID: 36092721, 25992900). PPIL2 is a large ~60 kDa (520 amino acids) protein that has a central cyclophilin-like domain and also has an N-terminal U-box domain that exhibits E3 ligase activity. Western detected PPIL2 at an apparent molecular mass of 60~70 kDa (PMID: 40338661, 29352246).

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