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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using PPIL1 Polyclonal antibody (15144-1-AP)

    WB analysis of NIH/3T3 using 15144-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPIL1 Polyclonal antibody (15144-1-AP)

    WB analysis of HeLa using 15144-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using PPIL1 Polyclonal antibody (15144-1-AP)

    WB analysis of A375 using 15144-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using PPIL1 Polyclonal antibody (15144-1-AP)

    IP experiment of NIH/3T3 using 15144-1-AP

    IP result of anti-PPIL1 (IP:15144-1-AP, 3ug; Detection:15144-1-AP 1:400) with NIH/3T3 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPIL1 Polyclonal antibody (15144-1-AP)

    IHC staining of human colon cancer using 15144-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPIL1 Polyclonal antibody (15144-1-AP)

    IHC staining of human colon cancer using 15144-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PPIL1 Polyclonal antibody (15144-1-AP)

    IF Staining of NIH/3T3 using 15144-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 15144-1-AP (PPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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PPIL1 Polyclonal antibody

PPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15144-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CGI 124, CGI-124, CYPL1, EC:5.2.1.8, hCyPX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, A375 cells, HeLa cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

15144-1-AP targets PPIL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIL1 fusion protein Ag7436 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase (cyclophilin)-like 1
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC003048
Gene Symbol PPIL1
Gene ID (NCBI) 51645
RRIDAB_2169603
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peptidyl-prolyl isomerase (PPIase) catalyzes the interconversion of a specific Pro-imide bond between the cis and trans conformations. PPIL1 (Peptidyl-prolyl cis-trans isomerase-like 1) is up-regulated in human colon cancer cells and an siRNA-mediated knockdown of PPIL1 resulted in a reduced number of viable cell (PMID: 20368803). Expression of PPIL1 was shown to be ubiquitous across all adult tissues (with highest expression in the adrenal gland, testis and heart). PPIL1 is also known to participate in the spliceosome; a complex and dynamic protein and RNA complex responsible for splicing introns from pre-mRNA (PMID: 33220177, 8978786).

Protocols

Product Specific Protocols
WB protocol for PPIL1 antibody 15144-1-APDownload protocol
IHC protocol for PPIL1 antibody 15144-1-APDownload protocol
IF protocol for PPIL1 antibody 15144-1-APDownload protocol
IP protocol for PPIL1 antibody 15144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Neuron

Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly.

Authors - Guoliang Chai
View Article