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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclophilin E Polyclonal antibody (15357-1-AP)

    WB analysis using 15357-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cyclophilin E Polyclonal antibody (15357-1-AP)

    WB analysis of HeLa using 15357-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclophilin E Polyclonal antibody (15357-1-AP)

    IF Staining of HeLa using 15357-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin E antibody (15357-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Cyclophilin E Polyclonal antibody

Cyclophilin E Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15357-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

PPIE, Cyclophilin 33, Cyclophilin-33, CYP 33, CYP33

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15357-1-AP targets Cyclophilin E in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7603

Product name: Recombinant human PPIE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC004898

Sequence: MATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV

相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase E (cyclophilin E)
Calculated molecular weight 33 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC004898
Gene Symbol Cyclophilin E
Gene ID (NCBI) 10450
RRIDAB_2878131
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).

Protocols

Product Specific Protocols
WB protocol for Cyclophilin E antibody 15357-1-APDownload protocol
IF protocol for Cyclophilin E antibody 15357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols