PPID Polyclonal antibody

Name: PPID Polyclonal antibody
Brand: Proteintech
SKU: 12716-1-AP

PPID Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12716-1-AP

Synonyms

Cyclophilin 40, Cyclophilin D, Cyclophilin related protein, CYP 40, CYP40, CYPD, peptidylprolyl isomerase D, PPIase D, PPID, Rotamase D

Validation Data Gallery

Applications:

AllWBIPIHCFC

WB analysis of mouse brain using 12716-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12716-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12716-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12716-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 12716-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12716-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12716-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12716-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12716-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12716-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12716-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 12716-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12716-1-AP
IP result of anti-PPID (IP:12716-1-AP, 4ug; Detection:12716-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human cervical cancer using 12716-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with PPID antibody included
IHC staining of mouse testis using 12716-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12716-1-AP (PPID antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PPID antibody included
IHC staining of human cervical cancer using 12716-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with PPID antibody included
FC experiment of HeLa using 12716-1-AP
1X10^6 HeLa cells were stained with 0.2ug PPID antibody (12716-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Cat No. 12716-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, A549 cells, human brain tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, Raji cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse testis tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 7 publications below
IF	See 1 publications below
CoIP	See 2 publications below

Product Information

12716-1-AP targets PPID in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPID fusion protein Ag3409 相同性解析による交差性が予測される生物種
Full Name	peptidylprolyl isomerase D
Calculated molecular weight	370 aa, 41 kDa
Observed molecular weight	38-41 kDa
GenBank accession number	BC030707
Gene symbol	PPID
Gene ID (NCBI)	5481
RRID	AB_2237551
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Protocols

Product Specific Protocols
WB protocol for PPID antibody 12716-1-AP	Download protocol
IHC protocol for PPID antibody 12716-1-AP	Download protocol
IP protocol for PPID antibody 12716-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB,CoIP	Cardiovasc Toxicol PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice. Authors - Tingting Wu View Article
human	WB	Mol Med Rep Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis. Authors - Da-Wei Liu View Article
human	WB	Eur J Pharmacol A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria. Authors - Yong-Cheng Ma View Article
human	WB	Dev Cell Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome Authors - Ji Geng View Article
rat	WB,IF	Bone Res Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40) Authors - Junqin Li View Article
mouse,rat	WB,CoIP	J Hazard Mater T-2 toxin induces mitochondrial dysfunction in chondrocytes via the p53-cyclophilin D pathway Authors - Fang-Fang Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPID Polyclonal antibody

PPID Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of Jurkat using 12716-1-AP

WB analysis of Jurkat using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of Raji using 12716-1-AP

WB analysis of rat brain using 12716-1-AP

WB analysis of A549 using 12716-1-AP

WB analysis of A549 using 12716-1-AP

WB analysis of A549 using 12716-1-AP

WB analysis of Raji using 12716-1-AP

WB analysis of MCF-7 using 12716-1-AP

WB analysis of MCF-7 using 12716-1-AP

WB analysis of NIH/3T3 using 12716-1-AP

IP experiment of mouse brain using 12716-1-AP

IHC staining of human cervical cancer using 12716-1-AP

IHC staining of mouse testis using 12716-1-AP

IHC staining of human cervical cancer using 12716-1-AP

FC experiment of HeLa using 12716-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice.

Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.

A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria.

Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome

Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40)

T-2 toxin induces mitochondrial dysfunction in chondrocytes via the p53-cyclophilin D pathway