Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HuH-7 cells, HEK-293 cells, mouse heart tissue |
| Positive IHC detected in | human heart tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
10287-2-AP targets CYPC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0209 Product name: Recombinant human CYPC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-212 aa of BC002678 Sequence: RLLLPLVLCVGLGALVFSSGAEGFRKRGPSVTAKVFFDVRIGDKDVGRIVIGLFGKVVPKTVENFVALATGEKGYGYKGSKFHRVIKDFMIQGGDITTGDGTGGVSIYGETFPDENFKLKHYGIGWVSMANAGPDTNGSQFFITLTKPTWLDGKHVVFGKVIDGMTVVHSIELQATDGHDRPLTNCSIINSGKIDVKTPFVVEIADW 相同性解析による交差性が予測される生物種 |
| Full Name | peptidylprolyl isomerase C (cyclophilin C) |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC002678 |
| Gene Symbol | PPIC/CYPC |
| Gene ID (NCBI) | 5480 |
| RRID | AB_2169424 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45877 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclophilin C also named as peptidyl-prolyl cis-trans isomerase C(PPIC), is expressed in several bone marrow (BM) hematopoietic progenitors and in T‐cell precursors(PMID: 33864384). CYPC/PPIC catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. In addition, CYPC/PPIC is involved in many biological processes, including mitochondrial metabolism, REDOX and inflammation, as well as related diseases and conditions, such as ischemia-reperfusion injury, AIDS and cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYPC antibody 10287-2-AP | Download protocol |
| IHC protocol for CYPC antibody 10287-2-AP | Download protocol |
| WB protocol for CYPC antibody 10287-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem The isomerase active site of cyclophilin A is critical for hepatitis C virus replication.
| ||
J Biol Chem Depletion of Cyclophilins B and C Leads to Dysregulation of Endoplasmic Reticulum Redox Homeostasis. | ||
PLoS One Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules. | ||
ACS Chem Neurosci Furanoditerpenes from Spongia (Spongia) tubulifera Display Mitochondrial-Mediated Neuroprotective Effects by Targeting Cyclophilin D. | ||
Transl Oncol PPIC-labeled CAFs: Key players in neoadjuvant chemotherapy resistance for gastric cancer | ||
Int J Mol Sci Pharmacological Targeting of the ER-Resident Chaperones GRP94 or Cyclophilin B Induces Secretion of IL-22 Binding Protein Isoform-1 (IL-22BPi1). |
